Subject code BT3452 deals with the semester IV subject syllabus from Anna University 2021 relevant to affiliated institutions. In this article, we would like to introduce the syllabus of the BT3452 – Industrial Enzymology in B.Tech Biotechnology and Biochemical Engineering.
We like to provide the BT3452 – Industrial Enzymology syllabus for students to perform well academically. This article will assist the students in getting the required syllabus for each unit with different concepts. We include expert textbooks and references for students to avoid confusion when reading books. The following links will help you to select the subject you want to read. Hope this information is useful. Don’t forget to share this information with your friends.
If you want to know more about the syllabus of B.Tech Biotechnology and Biochemical Engineering Syllabus connected to an affiliated institution’s four-year undergraduate degree program. We provide you with a detailed Year-wise, semester-wise, and Subject-wise syllabus in the following link B.Tech Biotechnology and Biochemical Engineering Syllabus Anna University Regulation 2021.
Aim Of Concept:
To enable the students
- To learn enzyme reactions and its characteristics along with the production and purification process
- To give the student a basic knowledge concerning biotransformation reactions with the usage of enzymes
BT3452 – Industrial Enzymology Syllabus
Unit I: Introduction To Enzymes
Classification of enzymes. Mechanisms of enzyme action; concept of active site and energetics of enzyme substrate complex formation; specificity of enzyme action; principles of catalysis – collision theory, transition state theory; role of entropy in catalysis.
Unit II: Kinetics Of Enzyme Action
Kinetics of single substrate reactions; estimation of Michelis – Menten parameters, multisubstrate reactions – mechanisms and kinetics; turnover number; types of inhibition & models –substrate, product. Allosteric regulation of enzymes, Monod Changeux Wyman model, pH and temperature effect on enzymes & deactivation kinetics.
Unit III: Enzyme Immobilization And Biosensors
Physical and chemical techniques for enzyme immobilization – adsorption, matrix entrapment, encapsulation, cross-linking, covalent binding etc., – examples, advantages and disadvantages, design of enzyme electrodes and their application as biosensors in industry, healthcare and environment.
Unit IV: Purification And Characterization Of Enzymes From Natural Sources
Production and purification of crude enzyme extracts from plant, animal and microbial sources; methods of characterization of enzymes; development of enzymatic assays
Unit V: Biotransformation Applications Of Enzymes
Hydrolytic- Ester bond, Amide, Epoxides, Nitriles, Reduction reactions –aldehydes, Ketones, C=C, Oxidation reactions – Alkanes, Aromatic, Baeyer-Villiger, Enzymes in organic synthesis – esters, amide, peptide, Modified and Artificial Enzymes, Catalytic antibodies
Text Books:
- Trevor Palmer, Enzymes II nd Horwood Publishing Ltd
- Faber K, Biotransformations in Organic Chemistry, IV edition, Springer
References:
- Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc.
- James M. Lee, Biochemical Engineering, PHI, USA.
- James. E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
- Wiseman, Enzyme Biotechnology, Ellis Horwood Pub.
Related Posts On Semester – IV:
- MA3391 – Probability and Statistics
- GE3451 – Environmental Sciences and Sustainability
- BI3401 – Chemical Thermodynamics and Bio-Thermodynamics
- BT3451 – Analytical Techniques In Biotechnology
- BT3391 – Basic Industrial Biotechnology
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