Subject code BT3451 deals with the semester IV subject syllabus from Anna University 2021 relevant to affiliated institutions. In this article, we would like to introduce the syllabus of the BT3451 – Analytical Techniques In Biotechnology in B.Tech Biotechnology and Biochemical Engineering.
We like to provide the BT3451 – Analytical Techniques In Biotechnology syllabus for students to perform well academically. This article will assist the students in getting the required syllabus for each unit with different concepts. We include expert textbooks and references for students to avoid confusion when reading books. The following links will help you to select the subject you want to read. Hope this information is useful. Don’t forget to share this information with your friends.
If you want to know more about the syllabus of B.Tech Biotechnology and Biochemical Engineering Syllabus connected to an affiliated institution’s four-year undergraduate degree program. We provide you with a detailed Year-wise, semester-wise, and Subject-wise syllabus in the following link B.Tech Biotechnology and Biochemical Engineering Syllabus Anna University Regulation 2021.
Aim Of Concept:
To enable the students
- To have a fundamental knowledge about the Light spectrum, Absoprtion, Fluorescence, NMR, Mass spectroscopy
- To acquire knowledge on the different chromotographic methods for separation of biological products.
BT3451 – Analytical Techniques In Biotechnology Syllabus
Unit I: Introduction To Spectrometry
Properties of electromagnetic radiation- wave properties – components of optical instruments – Sources of radiation – wavelength selectors – sample containers – radiation transducers – Signal process and read outs – signal to noise ratio – sources of noise – Enhancement of signal to noise types of optical instruments – Principle of Fourier Transform optical Measurements.
Unit II: Molecular Spectroscopy
Molecular absorption spectrometry – Measurement of Transmittance and Absorbance – Beer’s law – Instrumentation – Applications -Theory of fluorescence and Phosphorescence – Instrumentation – Applications – Theory of Infrared absorption spectrometry – IR instrumentation – Applications – Theory of Raman spectroscopy – Instrumentation – applications
Unit III: Magnetic Resonance Spectroscopy And Mass Spectrometry
Theory of NMR – environmental effects on NMR spectra – chemical shift- NMR-spectrometers – applications of 1H and 13C NMR- Molecular mass spectra – ion sources – Mass spectrometer. Applications of molecular mass – Electron paramagnetic resonance- g values –instrumentation.
Unit IV: Separation Methods
General description of chromatography – Band broadening and optimization of column performance- Liquid chromatography – Partition chromatography – Adsorption chromatography – Ion exchange chromatography -size exclusion chromatography- Affinity chromatography- principles of GC and applications – HPLC – Capillary electrophoresis – Applications.
Unit V: Electro Analysis And Surface Microscopy
Electrochemical cells- Electrode potential cell potentials – potentiometry- reference electrode – ion selective and molecular selective electrodes – Instrument for potentiometric studies – Voltametry – Cyclic and pulse voltametry- Applications of voltammetry. Study of surfaces – Scanning probe microscopes – AFM and STM.
Text Books:
- Skoog, D.A. F. James Holler, and Stanky, R. Crouch “Instrumental Methods of Analysis”.Cengage Learning, 2007.
- Willard, Hobart, etal., “Instrumental Methods of Analysis”. VII th Edition, CBS, 1986.
- Fifield F.W., . Principles and Practice of Analytical Chemistry. Blackwell, Scientific Publishers,2016
References:
- Sharma, B.K. “Instrumental Methods of Chemical Analysis: Analytical Chemistry”, Krishna Prakashan Media (P) Ltd, 2014
- Haven, Mary C., etal., “Laboratory Instrumentation “. 4th Edition, Wiley India Pvt Ltd, 2010
- Philopose P.M. Analytical Biotechnology. Domihant Publishers & distributors, New Delhi, 2016.
Related Posts On Semester – IV:
- MA3391 – Probability and Statistics
- GE3451 – Environmental Sciences and Sustainability
- BI3401 – Chemical Thermodynamics and Bio-Thermodynamics
- BT3391 – Basic Industrial Biotechnology
- BT3452 – Industrial Enzymology
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