Subject code BT3362 deals with the semester III subject syllabus from Anna University 2021 relevant to affiliated institutions. In this article, we would like to introduce the syllabus of the BT3362 – Cell And Microbiology Laboratory in B.Tech Biotechnology and Biochemical Engineering.
We like to provide the BT3362 – Cell And Microbiology Laboratory syllabus for students to perform well academically. This article will assist the students in getting the required syllabus for each unit with different concepts. We include expert textbooks and references for students to avoid confusion when reading books. The following links will help you to select the subject you want to read. Hope this information is useful. Don’t forget to share this information with your friends.
If you want to know more about the syllabus of B.Tech Biotechnology and Biochemical Engineering Syllabus connected to an affiliated institution’s four-year undergraduate degree program. We provide you with a detailed Year-wise, semester-wise, and Subject-wise syllabus in the following link B.Tech Biotechnology and Biochemical Engineering Syllabus Anna University Regulation 2021.
Aim Of Concept:
- To demonstrate various techniques to learn the morphology, identification and propagation of cells and microbes.
- To learn the staining techniques and culturing of microorganism.
BT3362 – Cell And Microbiology Laboratory Syllabus
Experiments:
- Introduction, Laboratory Safety, Use of Equipment; Sterilization Techniques
- Microscopy – Working and care of Microscope, phase contrast and fluorescent microscopy
- Culture Media-Types and Use; Preparation of Nutrient broth and agar
- Culture Techniques, Isolation and Preservation of Cultures- Broth: flask, test tubes; Solid: Pour plates, streak plates, slants, stabs
- Identification of given plant, animal, bacterial cells and yeast/mould
- Staining Techniques Simple, Differential- Gram’s Staining, spore /capsule staining, Giemsa, and Leishman Staining
- Quantification of Microbes: Sampling and Serial Dilution; Bacterial count in Soil – TVC
- Effect of Disinfectants- Phenol Coefficient, Antibiotic Sensitivity Assay
- Osmosis and Tonicity and Tryphan Blue Assay
- Growth Curve in Bacteria and Yeast
- Staining for different stages of mitosis in AlliumCepa (Onion)
- Effect of pH, Temperature, UV radiation on Growth Bacteria
References:
- Cappuccino, J.G. and N. Sherman “Microbiology: A Laboratory Manual”, 4th Edition, Addison-Wesley, 1999.
- Collee, J.G. etal., “Mackie & McCartney Practical Medical Microbiology” 4th Edition, Churchill Livingstone, 1996Rickwood, D. and J.R. Harris “Cell Biology: Essential Techniques”, John Wiley, 1996.
- Davis, J.M. “Basic Cell Culture: A Practical Approach”, IRL, 1994.
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