Subject code BT3361 deals with the semester III subject syllabus from Anna University 2021 relevant to affiliated institutions. In this article, we would like to introduce the syllabus of the BT3361 – Biochemistry Laboratory in B.Tech Biotechnology and Biochemical Engineering.
We like to provide the BT3361 – Biochemistry Laboratory syllabus for students to perform well academically. This article will assist the students in getting the required syllabus for each unit with different concepts. We include expert textbooks and references for students to avoid confusion when reading books. The following links will help you to select the subject you want to read. Hope this information is useful. Don’t forget to share this information with your friends.
If you want to know more about the syllabus of B.Tech Biotechnology and Biochemical Engineering Syllabus connected to an affiliated institution’s four-year undergraduate degree program. We provide you with a detailed Year-wise, semester-wise, and Subject-wise syllabus in the following link B.Tech Biotechnology and Biochemical Engineering Syllabus Anna University, Regulation 2021.
Aim Of Concept:
- To learn and understand the principles behind the qualitative and quantitative estimation of biomolecules (proteins, carbohydrates, lipids, metabolites, etc.,) and laboratory analysis of the same in the body fluids.
BT3361 – Biochemistry Laboratory Syllabus
Experiments:
- General guidelines for working in biochemistry lab (theory)
- Units of volume, weight, density and concentration measurements and their range in biological measurements. Demonstration of proper use of volume and weight measurement devices.
- Accuracy, precision, sensitivity and specificity (theory)
- Preparation of buffer –titration of a weak acid and a weak base.
- Qualitative tests for carbohydrates – distinguishing reducing from non-reducing sugars and keto from aldo sugars.
- Quantitative method for amino acid estimation using ninhydrin – distinguishing amino from imino acid.
- Protein estimation by Biuret and Lowry’s methods.
- Protein estimation by Bradford and spectroscopic methods.
- Extraction of lipids and analysis by TLC.
- Estimation of nucleic acids by absorbance at 260 nm and hyperchromic effect (demo).
- Enzymatic assay: phosphatase from potato.
- Enzymatic assay: estimation of glucose by GOD-POD method after hydrolysis of starch with acid and specificity of the enzymatic method.
Text Books:
- Practical Biochemistry by R.C. Gupta and S. Bhargavan.
- Introduction of Practical Biochemistry by David T. Phummer. (II Edition)
References:
- Harpers Biochemistry Ed. R.K. Murray, D.K. Granner, P.A. Mayes and V.W.Rodwell, Appleton and Lange, Stanford, Conneticut.
- Textbook of Biochemistry with clinical correlations. Ed. Thomas M. Devlin. Wiley Liss Publishers.
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